Troponin T hs

Troponin T hs

Used for the quantitative determination of Troponin T hs in human plasma and serum via electrochemiluminescent immunoassay on the Roche Cobas automated analyzer.

Instrument: Roche Cobas

Specimen Type: Serum; EDTA Plasma; Lithium-Heparin Plasma

NOTE: Unable to report results for hemolyzed specimens.

Volume per test: 49uL (min volume 200uL)

Principle:

The total duration of this sandwich principle method is 18 minutes. During the first incubation, 50 μL of specimen, a biotinylated monoclonal cardiac troponin T‑specific antibody, and a monoclonal cardiac troponin T‑specific antibody labeled with a ruthenium complex react to form a sandwich complex. After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin during a second incubation. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are removed with ProCell/ProCell M. Application of a voltage to the electrode induces a chemiluminescent emission which is measured by a photomultiplier. Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve provided via the reagent barcode.

Testing Range: 3-10000 ng/L