Glucose Roche Cobas

Glucose Roche Cobas

Used for the quantitative determination of glucose in human serum via enzymatic in vitro assay on the Cobas automated analyzer.

Instrument: Roche Cobas

Specimen Type: Serum

Volume per test: 2uL (min volume 125uL)

Principle:

This UV test involves two additive steps: Specimen and R1, then addition of R2 which initiates the reaction:

Glucose + ATP → G-6-P + ADP

Glucose-6-phosphate dehydrogenase oxidizes glucose-6-phosphate in the presence of NADP to gluconate-6-phosphate. No other carbohydrate is oxidized. The rate of NADPH formation during the reaction is directly proportional to the glucose concentration and can be measured photometrically.

Testing Range: 0.1-41.6 mmol/L