Pro-Matrix Metalloproteinase 7 (MMP-7)

Pro-Matrix Metalloproteinase 7 (MMP-7)

Used for the determination of pro-Matrix Metalloproteinase 7 (MMP-7) concentration in human serum using R&D Systems Quantikine Human Total MMP-7 ELISA kit.

Instrument: Manual assay

Specimen Type: Serum

Volume per test: 2 x 100uL (min volume 300uL)

Principle:

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MMP-7 is pre-coated on the microplate. Standards and samples are pipetted into the wells, and MMP-7 is bound by the immobilized antibody. After washing away unbound substances, an enzyme-linked polyclonal antibody specific for human MMP-7 is added to the wells. Following a wash to remove unbound antibody-enzyme reagent, a substrate solution is added and colour develops in proportion to the amount of total MMP-7 (pro and/or active) bound in the initial step. The colour development is stopped and the intensity of the colour is measured.

Testing Range: 0.156-10 ng/mL